Abstract
Surface-enhanced Raman Scattering Enhancement Factors for RNA Mononucleotides on Silver Nanoparticles
Highlights
S URFACE-ENHANCED Raman scattering (SERS) spectroscopy is a powerful vibrational spectroscopy technique that allows for highly sensitive structural detection of low concentration molecules in vicinity to nanostructured metal surfaces.[1]
To study the enhancement efficiency of the nanoparticles varying in the stabilizing ion layer on the silver surface, which affect the adsorption of the molecules on the enhancing metal surface, silver nanospheres covered by chloride (Ag_Cl nsp) and citrate (Ag_cit nsp) ions were prepared
In a series of the tested silver nanoparticles, the Raman scattering of the mononucleotides was strongly enhanced from the chloride coated nanospheres, moderately from the citrate covered nanospheres, and poorly from the citrate stabilized nanostars
Summary
S URFACE-ENHANCED Raman scattering (SERS) spectroscopy is a powerful vibrational spectroscopy technique that allows for highly sensitive structural detection of low concentration molecules in vicinity to nanostructured metal surfaces.[1]. The prerequisite for the Raman scattering enhancement is adsorption of the molecules onto or near a roughened metal surface. In order to enable effective surface adsorption, various SERS active substrates have been developed, ranging from electrodes, metal island films, to commonly used metal colloids.[3] Colloidal suspensions seem to be attractive due to rather simple and inexpensive preparation procedures, average SERS enhancement up to 106 and fair reproducibility.[4] In addition, the maximal surface enhancement of the scattered radiation can be achieved by adjusting the colloidal nanoparticles properties. The majority of the metal nanoparticles have been made of gold (Au) and silver (Ag), both metals being characterized
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