Abstract
Foodborne infections caused by Staphylococcus aureus (S. aureus) represent a serious health risk to the general public. In this study, we proposed a surface-enhanced Raman spectroscopy (SERS) biosensor based on a signal-amplification sandwich-type system for detecting and quantifying S. aureus. Herein, the SERS response of SiO2-coated Au@Ag was studied using Raman signals from Au@Ag@SiO2 nanoparticles and van grafted PDMS for the target acquisition. It has been shown that SiO2 coated Au@Ag exhibited a robust and strong response to SERS. Therefore, the combination of vancomycin (Van) and polydimethylsiloxane (PDMS) allowed a more efficient capture of S. aureus, resulting in improved repeatability through simplified separation procedures. Consequently, as a result of the presence of S. aureus targets, sandwich-type conjugate structures between the capture and signal units, as well as synergistic Raman amplification, were observed. Based on optimized conditions, a good linear correlation (y = 1087.86x - 540.75, R2 = 0.9958) in a wide dynamic range of 38–3.8 × 107 cfu·mL−1 was achieved with a limit of detection of 2 cfu·mL−1. The accuracy of the sensors was evaluated using a standard addition technique, and the results were satisfactory. Finally, the overall biosensor demonstrated potential application for monitoring S. aureus with good recoveries.
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