Abstract

Gold nanoparticles (AuNPs) are attractive structures for biosensing, most due to different properties at nanoscale and biocompatibility. Localized surface plasmon resonance (LSPR) is one of these properties; LSPR enable the electromagnetic field enhancement closer to metallic surface, which allows surface-enhanced spectroscopies, like surface enhanced fluorescence (SEF). In this study, an immuno-biosensor based on gold nanorods (AuNRs) and SEF was constructed for simple and fast analysis to detect albumin antibody (anti-BSA) using antigen–antibody (anti-BSA/BSA) interaction as the biorecognition model. AuNRs were presented in two distinct configurations, in suspension (S-AuNRs) and adsorbed on glass slides (AuNRs-chip), and the detection was performed through an extrinsic method, wherein the SEF signal of a reporter molecule (IR-820 cyanine-type dye) was monitored. The analyte detection was evidenced by SEF mapping, where the average signal in the presence of anti-BSA was three times more intense than for the assay in the absence of analyte. A digital protocol was proposed to simplify the spectroscopic data analysis and reduce the intensity variability; in this protocol the number of positive events in the presence of anti-BSA is much larger (around two times) compared to the absence of analyte. The AuNRs based SEF immuno-biosensor allowed an efficient and simple analysis with specific biorecognition and may contribute as an efficient spectroscopy platform for immuno-biosensing.

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