Surface effects in preparation of cell‐size liposomes

Abstract

Effects of surface type and area were shown to be important in the yield of cell‐size liposomes, but not in determining their size. The liposomes were prepared by dissolving lipids in a chloroform‐methanol solution and then evaporating the solvent under nitrogen in the presence of glass beads. After evaporation of the solvent, which was rapid due to the increased surface area, the dried lipids were then swollen in water at high temperatures (higher than the phase transition of the lipids), which led to formation of giant liposomes. The number of liposomes prepared in the presence of pyrex glass beads, which increase more than 100‐times the surface area of lipid‐glass contact, is more than 5‐times larger than in the control experiments without glass beads. The yield of liposomes in the presence of another type of glass bead was almost the same as in the control experiments. These effects may be due to long‐ and short‐range intermolecular interactions in the glass/water/lipid system.