Abstract
The inhibition of lipid absorption in digestive system is an effective strategy in obesity treatment. Pancreatic Lipase inhibitor (PLI) peptides, as novel anti-obesity agents, are currently under focus. In the present study we used a yeast surface display system to express PLI peptides in probiotic yeast Saccharomyces boulardii. Using bioinformatic approaches, two PLIs were chosen and accordingly, two different gene cassettes encoding surface GFP-PLI fusion proteins were successfully expressed in S. boulardii. The results of in vitro PL inhibition assay indicated a significant dose-dependent effect for both recombinant S. boulardii-PLI1 and 2 with IC50 of 106 and 104 cells/ reaction, respectively. The biological activity of these recombinant probiotics was examined in a rat model of obesity. The oral administration of recombinant yeasts (109 yeast cells/rat/day) resulted in significant weight loss and reduced serum lipids in treated animals. Our results indicate the potential application of such engineered strains in treating obesity.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
