Abstract
The surface chemical composition, the physicochemical properties, and the adhesiveness of Azospirillum brasilense have been investigated during growth in Luria–Bertani* rich medium. The surface elemental composition obtained by X-ray photoelectron spectroscopy was converted into a molecular composition in terms of model constituents: proteins, polysaccharides, and hydrocarbon-like compounds. The protein content increased during growth, from 30 (exponential phase cells) to 50% (stationary phase cells), concomitantly with a decrease in the polysaccharide content, from 60 to 35%. These modifications were related to a change in cell surface hydrophobicity, i.e., to an increase of the water contact angle from 20 to 60°. No difference of electrophoretic mobility was detected between cells harvested in the exponential phase and cells harvested in the stationary phase. The increase of both cell surface protein concentration and cell surface hydrophobicity during growth was correlated with an increase of cell adhesiveness to model supports. This points to the involvement of cell surface proteins and cell surface hydrophobicity in the adhesion process.Key words: Azospirillum brasilense, surface composition, hydrophobicity, adhesiveness, X-ray photoelectron spectroscopy.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
