Surface charge affects foliar uptake, transport and physiological effects of functionalized graphene quantum dots in plants

Abstract

The present study focused on evaluating the effects of surface charge on foliar uptake, translocation and physiological response of graphene quantum dots (GQDs) in maize (Zea mays L.) plants. Here, maize seedlings were foliar exposed to 10 mg/L GQDs modified with positively charged amino functional groups (NH2-GQDs) and negatively charged hydroxyl functional groups (OH-GQDs) for 8 days, respectively. Positively charged NH2-GQDs adhered on the cuticle layer were approximately 2.1 times more than the negatively charged OH-GQDs due to the electrostatic attraction to plant cell wall with negative charge. Within the initial 5 days, most of the GQDs internalized into the leaves via stomatal opening were efficiently translocated to the vasculature and moved down to the roots. Thereafter, the enlargement of aggregation made the particle sizes approach and even exceed the pipe diameter of vascular bundle, thus limiting the leaf-to-root translocation of GQDs, especially for NH2-GQDs. Compared with positively charged NH2-GQDs, negatively charged OH-GQDs induced stronger inhibitory effect on photosynthesis, higher accumulation of malondialdehyde and stimulation to enzyme activities of superoxide dismutase, catalase, and peroxidase. Overall, our findings provide direct evidence for the influence of surface charge on foliar uptake, translocation, and physiological effects of GQDs in crop plants, and imply that foliar exposure of GQDs negatively impact plant photosynthesis and growth health.