Surface characteristics, cytotoxicity, and microbial adhesion of 3D-printed hybrid resin-ceramic materials for definitive restoration

Abstract

ObjectiveThis study investigated the surface properties, cytotoxicity, and microbial adhesion of 3D-printed specimens made from hybrid resin-ceramic materials intended for use in definitive crowns. MethodsDisc-shaped specimens were 3D-printed using six different hybrid resin-ceramic materials recommended for definitive restorations: Crowntec (CT), VarseoSmile Crown Plus (VS), Tera Harz TC-80DP Graphy (TH), C&B Permanent ODS (CB), Formlabs Permanent Crown (FP), and HeyGears (HG). Surface topography, surface roughness, and water contact angle values were measured for each material (n = 6). Cytotoxicity was assessed using direct contact and extract tests on human gingival fibroblasts (n = 4). Additionally, the adhesion of mixed oral bacteria to the surfaces of the specimens was evaluated by counting colony-forming units (CFUs) after a 2-hour incubation period (n=6). ResultsThe TH group exhibited significantly lower surface roughness (Ra: 0.28 ± 0.13 μm) compared to the other materials (CT: 1.87 ± 0.34 μm; VS: 1.13 ± 0.09 μm; CB: 2.91 ± 0.27 μm; FP: 2.50 ± 0.08 μm; HG: 1.50 ± 0.55 μm). The VS group had the highest water contact angle (129.5 ± 1.1°), indicating greater hydrophobicity, in contrast to the other groups (CT: 72.6 ± 2.1°; TH: 75.0 ± 0.3°; CB: 69.1 ± 0.2°; FP: 93.0 ± 1.6°; HG: 77.7 ± 0.3°). Cytotoxicity testing showed no harmful effects, as relative cell viability exceeded 70%, and lactate dehydrogenase (LDH) release remained below 30% for all materials. The TH specimens also demonstrated the lowest bacterial adhesion. ConclusionsThe surface characteristics of the tested resin-ceramic materials varied significantly, with TH showing the smoothest surface and the least bacterial adhesion. All materials were found to be non-toxic. Therefore, TH material has the potential to provide definitive restorations with less microbial adhesion. Clinical SignificanceThe type of resin-ceramic material significantly affects the surface properties of 3D-printed specimens. These findings are crucial for selecting the appropriate resin-ceramic material for definitive restorations.