Abstract
Light exerts a major influence on the reproductive functions of the female rat, and the presence of constant light results in an aberrant vaginal cycle, leading to infertility associated with polycystic ovary (PCO) syndrome. In the present communication, we have utilized scanning electron microscopy (SEM) to study changes on the surface epithelium of persistent estrous (PE) rats with PCO.Sprague-Dawely female rats were exposed to constant light for 50-150 days according to protocol that has been previously described. Rats in normal estrous, as determined by vaginal smears, were used as controls. Nembutal- anesthesized rats were perfused through the aorta with 2.5% gluteraldehyde in 0.1M sodium cacodylate buffer (pH 7.3). The mucosa of the upper third of the vagina from PE and control rats were microdissected, processed for SEM studies by a modified 0T0T0 technique, dehydrated, and critical point dried with C02- The specimens were examined in a Hitachi scanning electron microscope Model S-500.
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