Abstract
AbstractMicroarrays are a versatile platform for diagnostics and high‐throughput analysis. Carbohydrate microarrays are valuable tools to investigate interactions with other molecules since many glycans are involved in fundamental biological processes. A combined X‐ray photoelectron spectroscopy (XPS) and time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) surface analysis was used to investigate the basic steps in the production of carbohydrate microarrays. The preparation included coupling of a thiol‐terminated mannoside to maleimide‐functionalized glass surfaces derived from γ‐aminopropyl silane (GAPS) slides. XPS results clearly demonstrate successful chemical modification in each fabrication step, and ToF‐SIMS imaging revealed immobilized carbohydrates in the spotted regions of the final microarray. Copyright © 2010 John Wiley & Sons, Ltd.
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