Surface analyses of orthopedic fixation screws submitted different artificial rest soil.

Abstract

Event Abstract Back to Event Surface analyses of orthopedic fixation screws submitted different artificial rest soil. Rodrigo Franca1, Michelle Alfa2, Nancy Olson2, L’hocine Yahia3 and Edward Sacher4 1 University of Manitoba, Dental Biomaterials Research Lab, Canada 2 University of Manitoba, St-Boniface Hospital Research Center, Canada 3 École Polytechnique de Montréal, Laboratoire d’Innovation et d’Analyse de Bioperformance, Canada 4 École Polytechnique de Montréal, Regroupement Québecois de Matériaux de Pointe, Canada Implantable plates, screws and wires are frequently held in the surgical instrument tray, and are reprocessed every time the instruments are used. There have been questions raised about this practice but there is a lack of data to demonstrate organic residuals on reprocessed implantable plates, screws or wires. The objective of this study was to determine is surface analysis techniques could be used to detect such residual organic material on stainless steel malleolus screws. New malleolus screws were compared to ones that had been coated in purified lipopolysaccharide or Artificial Test Soil (ATS) containing lipopolysaccharide. X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and surface time-of flight secondary ion mass spectrometry (TOF-SIMS) were used to assess the fixation screws. Organic material was visualized on the LPS and ATS-LPS inoculated screws but not on the new unsoiled screws. This was further supported by the peaks observed at masses between 40 – 100 D on TOF-SIMS spectra of the LPS inoculated and ATS-LPS inoculated screws. After deconvolution of high resolution XPS analyses, the LPS inoculated screws showed a carbon and oxygen oxidized state whereas the ATS-LPS inoculated screws showed predominantly C-NO (nitroso). In conclusion, our data demonstrated that surface analysis can detect organic residuals present in fixation screws. The XPS data confirmed that LPS reacted predominantly with positively charged surface metallic ions (Fe+ and Cr+) whereas proteins reacted with the surface oxide layer of fixation screws forming C-NO. The application of these surface analysis techniques will be helpful in determining if the reprocessing of such items results in an accumulation of organic material that might lead to aseptic loosening when implanted. Keywords: biomaterial Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Surface and interfacial characterization Citation: Franca R, Alfa M, Olson N, Yahia L and Sacher E (2016). Surface analyses of orthopedic fixation screws submitted different artificial rest soil.. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02947 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Rodrigo Franca Michelle Alfa Nancy Olson L’hocine Yahia Edward Sacher Google Rodrigo Franca Michelle Alfa Nancy Olson L’hocine Yahia Edward Sacher Google Scholar Rodrigo Franca Michelle Alfa Nancy Olson L’hocine Yahia Edward Sacher PubMed Rodrigo Franca Michelle Alfa Nancy Olson L’hocine Yahia Edward Sacher Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.