Surface air discharge used for biomedicine: the positive correlation among gaseous NO3, aqueous O2 −/ONOO− and biological effects

Abstract

Surface air discharge has been extensively reported to have strong antibacterial and anticancer effects, and these biological effects are more or less attributed to the short-lived aqueous reactive species produced by the air plasma. Insight into the generation mechanism for short-lived species is a key bottleneck in elucidating the antimicrobial and anticancer effects. Although the numerical study has predicted that the dissolution of gaseous NO3 plays a crucial role, but so far without experimental validation. In view of this, cavity ring-down spectroscopy is adopted in this paper to measure the NO3 spatial distribution between the surface air discharge and the solutions to be treated, and the concentrations of short-lived species in the solutions after plasma treatment are also measured for different discharge modes. A simplified chemical pathway for the conversion of gaseous NO3 to aqueous ONOOaq −/ONOOHaq and O2aq − is proposed. Moreover, the inactivation effects for Escherichia coli and A549 lung cancer cells treated by the plasma-activated solutions are measured for different concentrations of short-lived species, and the key species for sterilization and anticancer are identified by chemical scavengers. Finally, a positive correlation chain is found among the inactivation effect, the concentrations of aqueous ONOOaq −/ONOOHaq and O2aq −, and the density of gaseous NO3, implying that NO3 might be very important for the production of aqueous short-lived reactive species as well as the biological effects induced by plasma-activated solutions.