Surface affinity chromatographic separation of blood cells : IV. Relationship between surface hydrophobicity of human peripheral blood cells and their retention behaviour on polyethylene glycol 20M-bonded sepharose columns

Abstract

The partition behaviour of human peripheral blood cells was studied in dextran T40-polyethylene glycol (PEG) 6000 two-phase systems with and without PEG 6000 palmitate as a hydrophobic ligand at pH 7.5 and at the isoelectric points for the four kinds of cells. Δlog K, where K is the partition coefficient, is taken as a measure of the hydrophobic interactions between the cell surface and the palmitoyl residue. Its value is dependent on the concentration of palmitate in the partition systems and on the kinds of cells, increasing in the order platelets, granulocytes, lymphocytes and erythrocytes. A linear relationship was found between the retention volumes of these cells, except that of erythrocytes, on a PEG 20M-Sepharose column and their Δlog K values determined in the presence or absence of sodium chloride both at the isoelectric points and at pH 7.5.