Abstract
For gel permeation chromatography of humic acids, use of a neutral phosphate buffer containing 2 M urea is recommended. It was determined that the excluded fraction increased with an increase in standing time of sample in buffer solution before injection into the column. From the gel permeation chromatography of mixed samples of excluded and retarded fractions and measurement of the surface tension of each fraction, it was concluded that the reason is that large humic molecules tend to associate with small ones to form micelle-like aggregates. Various types of humic acids were subjected to the measurement of surface tension and gel permeation chromatography. Humic acid containing a large amount of excluded fraction showed high surface activity. The relationships between surface activity and structural properties of humic acid are discussed.
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