Abstract
The conventional sodium dodecylbenzenesulfonate (NaDBS) has been converted into an efficient and nontoxic anionic surface-active ionic liquid, cholinium dodecylbenzenesulfonate (Cho[DBS]). We have investigated its self-assembling behavior, interaction with the enzyme cellulase, and ecotoxicity. The surface-active properties at the air–liquid interface and the aggregation behavior of Cho[DBS] in water have been determined using tensiometry, isothermal titration calorimetry (ITC), conductometry, and dynamic light scattering (DLS). The enzyme activity was observed using dinitro salicylic acid analysis. The enhanced enzyme activity was explained through active-site exfoliation and structural constancy of cellulase in the micellar medium using the results from fluorescence, circular dichroism, DLS, and ITC. The nontoxic nature was confirmed by toxicity analysis on the freshwater microalgae Scenedesmus sp.
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