Sur les inclusions paracristallines des cellules nourricières de la galle provoquée par Diplolepis rosae L. sur Rosa canina L.

Abstract

Numerous crystalline inclusions are found in the nutritive cells lining the larval cavities of the galls induced by Diplolepis rosae L. on Rosa canina L. Electron microscopic investigations show that these intracytoplasmic inclusions consist of staggered, closely parallel, and slightly wavy filaments. In each filament three fibrils can be distinguished inside a less electron-dense matrix. The different aspects that the filaments may present according to the section plans are studied with an electron microscope equipped with a goniometric stage. Enzymatic digestion of ultrathin sections show that the paracrystals are essentially composed of proteins. This conclusion is sustained by the results of an ultrastructural autoradiographic study using tritiated aminoacids. The physiological significance of these paracrystals is discussed: their presence is probably related to a larval action which stimulates proteosynthesis in the cells surrounding the consumed ones.