Supraphysiological Levels of Testosterone Induce Vascular Dysfunction via Activation of the NLRP3 Inflammasome.

Juliano Vilela Alves,Camila André Pereira,Rafael Menezes Da Costa,Núbia Souza Lobato,Aline Garcia Fedoce,Carlos Alberto Aguiar Silva,Rita C Tostes,Fernando Silva Carneiro

doi:10.3389/fimmu.2020.01647

Juliano Vilela Alves, Camila André Pereira + Show 6 more

Open Access

https://doi.org/10.3389/fimmu.2020.01647

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Abstract

Background: Both supraphysiological and subphysiological testosterone levels are associated with increased cardiovascular risk. Testosterone consumption at supraphysiological doses has been linked to increased blood pressure, left ventricular hypertrophy, vascular dysfunction, and increased levels of inflammatory markers. Activation of the NLRP3 inflammasome contributes to the production of proinflammatory cytokines, leading to cardiovascular dysfunction. We hypothesized that supraphysiological levels of testosterone, via generation of mitochondrial reactive oxygen species (mROS), activates the NLRP3 inflammasome and promotes vascular dysfunction.Methods: Male, 12 week-old C57Bl/6J (WT) and NLRP3 knockout (NLRP3−/−) mice were used. Mice were treated with testosterone propionate [TP (10 mg/kg) in vivo] or vehicle for 30 days. In addition, vessels were incubated with testosterone [Testo (10−6 M, 2 h) in vitro]. Testosterone levels, blood pressure, vascular function (thoracic aortic rings), pro-caspase-1/caspase-1 and interleukin-1β (IL-1β) expression, and generation of reactive oxygen species were determined.Results: Testosterone increased contractile responses and reduced endothelium-dependent vasodilation, both in vivo and in vitro. These effects were not observed in arteries from NLRP3−/− mice. Aortas of TP-treated WT mice (in vivo), as well as aortas from WT mice incubated with testo (in vitro), exhibited increased mROS levels and increased caspase-1 and IL-1β expression. These effects were not observed in arteries from NLRP3−/− mice. Flutamide [Flu, 10−5 M, androgen receptor (AR) antagonist], carbonyl cyanide m-chlorophenyl hydrazone (CCCP, 10−6 M, mitochondrial uncoupler) and MCC950 (MCC950, 10−6 M, a NLRP3 receptor inhibitor) prevented testosterone-induced mROS generation.Conclusion: Supraphysiological levels of testosterone induce vascular dysfunction via mROS generation and NLRP3 inflammasome activation. These events may contribute to increased cardiovascular risk.

Highlights

Epidemiological studies have shown that men are at higher risk of cardiovascular disease than women, and sex steroids seem to contribute, at least in part, to this increased risk [1, 2]
To address whether supraphysiological levels of testosterone promote vascular dysfunction via NLRP3 inflammasome, WT and NLRP3−/− mice were treated with testosterone propionate (TP) (10 mg/kg for 30 days) or vehicle
Supraphysiological levels of testosterone in the TP groups were confirmed by comparison with vehicle-treated mice

Summary

Introduction

Epidemiological studies have shown that men are at higher risk of cardiovascular disease than women, and sex steroids seem to contribute, at least in part, to this increased risk [1, 2]. Testosterone has an important role in the regulation of cardiac function [11, 12] It affects cardiac contractility and relaxation, and cardiomyocyte repolarization. Testosterone modulates immune/inflammatory responses, displaying protective effects against atherosclerosis [13], and stimulating in vivo leukocyte-endothelial cell interactions, and contributing to increased leukocyte rolling and adhesion in male spontaneously hypertensive rats [14]. Both supraphysiological and subphysiological testosterone levels are associated with increased cardiovascular risk. We hypothesized that supraphysiological levels of testosterone, via generation of mitochondrial reactive oxygen species (mROS), activates the NLRP3 inflammasome and promotes vascular dysfunction

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