Suppressor of cytokine signaling 1 regulates the neuronal differentiation of C17.2 neuronal stem cells

Abstract

Objective To investigate the role of suppressor of cytokine signaling 1 (SOCS1) in regulating the differentiation of C17.2 neuronal stem cells (NSCs).Methods C17.2 NSCs were transfected by Lentiviral vector which included objective gene-SOCSl.The experiment was individed into LV-SOCS1 group,LV group and PBS group.The morphological change of C17.2NSCs after nransfection was observed by light microscopy.The expression of Nestin,microtubule-associated proteins 2 (MAP2),β-tubulinⅢ,myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP) in C17.2 NSCs was detected by immunocytochemistry,Western blotting and reverse transcription-polymerase chain reaction (RT-PCR).The protein expression of β-tubulinⅢ in C17.2 NSCs was examined by immunocytochemistry and Western blotting.Results An decreased expression of Nestin and an increased expression of MAP2 in LV-SOCS1 group was found as compared with LV group and PBS group after transfection (P ＜ 0.01).Some C17.2 NSCs in LV-SOCS1 group underwent prominent neuronal morphological changes after transfection and almost all the cells with morphological changes were β-tubulin Ⅲ-positive,and the other two groups had less cells with morphological changes and β-tubulin Ⅲ-positive (LV-SOCS1 group:10.05％,LV group:0.71％,PBS group:0.65％) (P＜0.01).The β-tubulin lⅢ expression in LV-SOCS1 group was significantly higher than in other two groups (P ＜0.01).Conclusion SOCS1 may play a role in promoting the neuronal differentiation of C17.2 NSCs. Key words: Suppressor of cytokine signaling 1 ; Neuronal stem cells; Neuron; Differentiation