Suppressive role on bacterial resistance of CD10+Pax5+cells in alcohol use disorder patients

Abstract

Abstract Alcohol use disorder (AUD) patients are the immunocompromised hosts and these patients have an increased risk of several opportunistic infections. CD10+Pax5+cells are included in lineage negative cells which have been described as suppressor cells for host antibacterial defenses. In this study, we examined the immunological role of CD10+Pax5+cells in AUD patients. CD10+Pax5+cells were isolated from peripheral blood of AUD patients and healthy donors (HD) and analyzed for retinoic acid receptor (RAR) expression by flow cytometry. These cells (1 x 106cells/ml) were cultured with 10 μg/ml of retinoic acid (RA) for 24 hours. After washing, culture fluids of these cells for 24 hours were harvested. Monocytes (1 x 106cells/ml) isolated from HD, were stimulated with culture fluids from CD10+Pax5+cells after the RA stimulation and the bactericidal activity against Enterococcus faecalis was assayed by colony counting methods. In the results, the number of CD10+Pax5+cells was same between AUD patients and HD. CD10+Pax5+cells from HD were expressed RAR (63.7 ± 2.19%), but those of AUD patients were not (12.4 ± 3.31%). When monocytes were stimulated with culture fluids of CD10+Pax5+cells after the RA stimulation from HD, the bactericidal activity of these cells was 62.75 ± 3.78%. However, the bactericidal activity of same monocytes which were stimulated with culture fluids of CD10+Pax5+cells after the RA stimulation from AUD patients, was suppressed(5.5 ± 3.15%). In conclusions, through the production of some soluble factors active against killing functions of monocytes, CD10+Pax5+cells increase the susceptibility of AUD patients to opportunistic infections.