Suppressive Effect of an Isoflavone Fraction on Tumor Necrosis Factor-α-Induced Interleukin-8 Production in Human Intestinal Epithelial Caco-2 Cells

Abstract

This study demonstrates the effect of soybean components on the tumor necrosis factor-alpha (TNF-alpha)-induced production of interleukin-8 (IL-8), one of the major inflammatory chemokines, in intestinal epithelial-like Caco-2 cells. Among the soybean components, an isoflavone fraction (IFF) suppressed the TNF-alpha-induced IL-8 secretion by Caco-2 cells in a dose-dependent manner, whereas a soyasaponin fraction and soypeptide fraction had no significant effect on TNF-alpha-induced IL-8 secretion. The IL-8 secretion induced by hydrogen peroxide and by IL-1beta was not suppressed by IFF, suggesting that the inhibitory effect of isoflavone was specific for the TNF-alpha-induced regulation of IL-8. The increased expression of IL-8 mRNA by TNF-alpha was almost completely suppressed by IFF. Furthermore, the transcriptional activity of the human IL-8 promoter was increased by the TNF-alpha treatment, and IFF significantly suppressed the IL-8 promoter activity. These results indicate that IFF suppressed TNF-alpha-induced IL-8 production at the transcriptional level in human intestinal Caco-2 cells, suggesting IFF of soybean as a promising food component for preventing intestinal inflammation such as inflammatory bowel disease.