Abstract
The EtOAc extract from rice (Oriza sativa cv. Hinohikari) husk showed a suppressive effect on umu gene expression of the SOS response in Salmonella typhimurium TA1535/pSK1002 against the mutagen, Trp-P-1, which requires liver metabolizing enzyme. To obtain the suppressive compound, the EtOAc extract was fractionated by SiO2 column chromatography using umu test as a bioassay guide. Suppressive compound was isolated and identified as momilactone A (1) by EIMS, IR, 1H- and 13C-NMR spectroscopy. Compound 1 inhibited of the SOS-inducing activity of Trp-P-1 in the umu test. Gene expression was suppressed by 32.6% at less than 0.60 mM. Compound 1 was assayed with activated Trp-P-1. The suppressive effect of Compound 1 was decreased compared with that of Trp-P-1. Furthermore, 1 was assayed with another mutagens, such as MeIQ, activated MeIQ, furylfuramide (AF-2), MNNG, and UV-irradiation. Compound 1 showed greater suppressive effect on AF-2-inducing SOS response than other mutagens.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
