Abstract

Suppression Subtractive Hybridization in Penaeid Prawns: An Approach in Identifying Diseases and Differentially Expressed Genes

Highlights

  • Alteration in gene transcripts is related to a wide spectrum of physiological and pathological changes in cells

  • The identification of differential gene expression can be used to understand biological processes at a molecular level.[1,2,3,4]. Various techniques such as Expressed Sequence Tags (EST), differential display, Serial Analysis of Gene Expression (SAGE), cDNA microarray, and subtractive hybridization have been developed to analyse differential gene expressions in any given sample.[5,6]

  • The suppression subtractive hybridization (SSH) combined with EST,[51] differential hybridization,[22,24] mirror orientation selection[52] or microarray analysis[53,54,55] has been used to elucidate genes involved in the immune response of penaeid prawns during white spot syndrome virus (WSSV) infection

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Summary

Introduction

Alteration in gene transcripts is related to a wide spectrum of physiological and pathological changes in cells. The common sequences of both samples are subtracted leaving a cDNA population enriched for sequence preferentially expressed in the tester but absent in the driver.[13,16,19] in the SSH method, target cDNA is selectively amplified, while an undesirable sequence is simultaneously suppressed during PCR amplification.

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