Suppression of Protein Kinase C Is Associated with Inhibition of PYK2 Tyrosine Phosphorylation and Enhancement of PYK2 Interaction with Src in Thrombin-Activated Platelets

Abstract

Blood platelets have recently been shown to express PYK2, a nonreceptor tyrosine kinase belonging to the FAK gene family. In this study, we examined the involvement of protein kinase C (PKC) in PYK2-related responses in human platelets. While PYK2 tyrosine phosphorylation induced by thrombin was inhibited by preincubation of platelets with PKC inhibitors, staurosporine and Ro31-8220, PYK2 association with Src was markedly enhanced under the same conditions. Platelet intracellular Ca 2+ mobilization induced by thrombin was hardly inhibited by these PKC inhibitors. p130 Cas is a docking protein that associates with FAK or PYK2 through the SH3 domain. Although we identified p130 Cas in platelets for the first time, this docking protein failed to interact with PYK2. These results suggest that PKC activation (but not Ca 2+ mobilization) is involved in PYK2 tyrosine phosphorylation and that PYK2 associates with Src without PYK2 tyrosine phosphorylation or p130 Cas involvement in platelets.