Abstract
Transport of bioactive cargo of microvesicles (MVs) into target cells can affect their fate and behavior and change their microenvironment. We assessed the effect of MVs derived from human immortalized mesenchymal stem cells of adipose tissue-origin (HATMSC2-MVs) on the biological activity of the ovarian cancer cell lines ES-2 (clear cell carcinoma) and OAW-42 (cystadenocarcinoma). The HATMSC2-MVs were characterized using dynamic light scattering (DLS), transmission electron microscopy, and flow cytometry. The anti-tumor properties of HATMSC2-MVs were assessed using MTT for metabolic activity and flow cytometry for cell survival, cell cycle progression, and phenotype. The secretion profile of ovarian cancer cells was evaluated with a protein antibody array. Both cell lines internalized HATMSC2-MVs, which was associated with a decreased metabolic activity of cancer cells. HATMSC2-MVs exerted a pro-apoptotic and/or necrotic effect on ES-2 and OAW-42 cells and increased the expression of anti-tumor factors in both cell lines compared to control. In conclusion, we confirmed an effective transfer of HATMSC2-MVs into ovarian cancer cells that resulted in the inhibition of cell proliferation via different pathways, apoptosis and/or necrosis, which, with high likelihood, is related to the presence of different anti-tumor factors secreted by the ES-2 and OAW-42 cells.
Highlights
Today, ovarian cancer is one of the most dangerous types of cancer in women
The results showed a significant increase in mean fluorescence intensity (MFI) in the ES-2 and OAW-42 cell lines treated with HATMSC2-MVs for both the ratios of 5:1 and 10:1 compared to the control groups (p < 0.001)
We investigated whether MVs derived from human immortalized Mesenchymal stem/stromal cells (MSCs) of adipose tissue origin may represent a new form of supportive therapy in ovarian cancer treatment
Summary
Ovarian cancer is one of the most dangerous types of cancer in women. This is associated with a lack of screening tests and late diagnosis. Various ovarian cancer therapies are used depending on the histological type of ovarian cancer, its stage, and the patient’s predisposition. An extremely important field in oncology is research focused on cancer stem cells (CSCs). Cancer stem cells constitute a small population of tumor cells and play an important role in metastasis. These cells are resistant to widely used drugs, which often leads to tumor recurrence [3]. A search for effective factors is needed that inhibit the biological activity of CSCs
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