Suppression of outward K+ currents by activating dopamine D1 receptors in rat retinal ganglion cells through PKA and CaMKII signaling pathways

Abstract

Dopamine plays an important role in regulating neuronal functions in the central nervous system by activating the specific G-protein coupled receptors. Both D1 and D2 dopamine receptors are extensively distributed in the retinal neurons. In the present study, we investigated the effects of D1 receptor signaling on outward K+ currents in acutely isolated rat retinal ganglion cells (RGCs) by patch-clamp techniques. Extracellular application of SKF81297 (10μM), a specific D1 receptor agonist, significantly and reversibly suppressed outward K+ currents of the cells, which was reversed by SCH23390 (10μM), a selective D1 receptor antagonist. We further showed that SKF81297 mainly suppressed the glybenclamide (Gb)- and 4-aminopyridine (4-AP)-sensitive K+ current components, but did not show effect on the tetraethylammonium (TEA)-sensitive one. Both protein kinase A (PKA) and calcium/calmodulin-dependent protein kinase II (CaMKII) signaling pathways were likely involved in the SKF81297-induced suppression of the K+ currents since either Rp-cAMP (10μM), a cAMP/PKA signaling inhibitor, or KN-93 (10μM), a specific CaMKII inhibitor, eliminated the SKF81297 effect. In contrast, neither protein kinase C (PKC) nor mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) signaling pathway seemed likely to be involved because both the PKC inhibitor bisindolylmaleimide IV (Bis IV) (10μM) and the MAPK/ERK1/2 inhibitor U0126 (10μM) did not block the SKF81297-induced suppression of the K+ currents. These results suggest that activation of D1 receptors suppresses the Gb- and 4-AP-sensitive K+ current components in rat RGCs through the intracellular PKA and CaMKII signaling pathways, thus modulating the RGC excitability.