Suppression of lipopolysaccharide-induced activation of RAW 264.7 macrophages by Se-methylseleno-l-cysteine

Abstract

Se-methylseleno-l-cysteine (l-SeMC) is a natural source of organic selenium for humans. Although it has a structure similar to that of l-Cysteine (l-Cys), its anti-inflammatory properties and possible underlying mechanisms have not been explored. Here, we compared the anti-inflammatory activities of inorganic selenium (selenite), l-Cys, and l-SeMC in lipopolysaccharide (LPS)-activated RAW 264.7 murine macrophages and focused on the related molecular and biochemical events. The results showed that, anti-inflammatory activity of l-SeMC was much stronger compared to both individual l-Cys treatment and l-Cys/selenite combinations. The organic form of selenium may play a crucial role in the effects of l-SeMC. Further study confirmed that l-SeMC suppressed the RNA expression of iNOS, TNF-α, IL-1β, IL-6, COX-2, and MMP-9, as well as the release of NO, TNF-α, IL-6, IL-12p70, COX-2, and PGE2 from LPS-activated RAW264.7 macrophages in a concentration-dependent manner. Moreover, l-SeMC prevented LPS-induced changes in cell morphology. l-SeMC concentrations between 50 and 200 μM exhibited an anti-inflammatory effect closed to that exhibited by 20 μM dexamethasone. Our results demonstrated that l-SeMC effectively inhibited the activation of RAW 264.7 macrophages induced by LPS, and suggested that l-SeMC could be a potential functional food component for the prevention or treatment of inflammatory diseases.