Year-end Sale % OFF 
Abstract
The Galectins are a family of proteins that share an affinity for Beta-galactoside containing glycoconjugates. Galectin-3 has been implicated in tumor progression and metastasis. It is also known that in prostate, ovarian and breast cancer, down regulation of Galectin-3 is associated with malignancy. Kaposi's sarcoma (KS) is characterized as an angioproliferative tumor of vascular endothelial cells and produces rare B cell lymphoproliferative diseases in the form of primary effusion lymphomas (PEL) and some forms of Multicentric Castleman's Disease (MDC). Kaposi's Sarcoma-Associated Herpesvirus also known as KSHV or Human Herpesvirus type 8 (HHV8) is the etiological agent of KS. We have observed suppression of galectin-3 expression in Kaposi's sarcoma (KS). Here we demonstrate that galectin-3 protein expression is down-regulated 20-fold in KSHV infected dermal microvascular endothelial cells (DMVEC) cells. We show loss of Galectin-3 staining by dual labeled immunofluorescence in latency associated nuclear antigen (LANA) positive spindle cells. We also find reduced levels of galectin-3 expression in LANA positive spindle cell regions in archival KS tissue. There is also transcriptional suppression of Galectin-3 message in KSHV infected DMVEC cells compared to mock infected controls. We demonstrate that KSHV vFLIP is the likely viral gene that targets galectin-3 downregulation in HeLa cells. In pleural effusion lymphoma cell lines (PEL), we observe different levels of galectin-3 expression associated with varying levels of KSHV replication. The search for novel host cell factors that may contribute to the overall pathogenesis of KS is essential for early detection of KS and development of innovative therapies for treatment.
Highlights
phlettmpe:/n/wt>wmeePnrtorcale.ceodmin/gcsoonftethnet/p1d1ft/h17In5t0e-r9n3a7ti8o-n4a-Sl 2C-oinnfofe.prednf MAIDS and Other Acquired Immunodeficiencies (ICMAOI): Basic, Epidemiologic, and Clinical Research Publication of this supplement was made possible with support from the Office of HIV and AIDS Malignancy, National Cancer Institute, National Institutes of Health. Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here.
Kaposi's Sarcoma-Associated Herpesvirus known as KSHV or Human Herpesvirus type 8 (HHV8) is the etiological agent of Kaposi's sarcoma (KS)
We demonstrate that galectin-3 protein expression is downregulated 20-fold in KSHV infected dermal microvascular endothelial cells (DMVEC) cells
Summary
phlettmpe:/n/wt>wmeePnrtorcale.ceodmin/gcsoonftethnet/p1d1ft/h17In5t0e-r9n3a7ti8o-n4a-Sl 2C-oinnfofe.prednf MAIDS and Other Acquired Immunodeficiencies (ICMAOI): Basic, Epidemiologic, and Clinical Research Publication of this supplement was made possible with support from the Office of HIV and AIDS Malignancy, National Cancer Institute, National Institutes of Health. Meeting abstracts – A single PDF containing all abstracts in this Supplement is available here.. Suppression of galectin-3 in KSHV infected DMVEC cells and Kaposi's sarcoma: implications for tumorigenesis Address: 1Meharry Medical College, School of Medicine, Center for AIDS Health Disparities Research and Department of Microbial Pathogenesis and Immune Response, Nashville, Tennessee, USA and 2Department of Viral Oncology and Pharmacology, Molecular Virology Laboratories, Sidney-Kimmel Cancer Research Center, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA * Corresponding author from 11th International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI): Basic, Epidemiologic, and Clinical Research Bethesda, MD, USA.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
