Abstract
Surface localization of a major autolysin (atl gene product, ATL) on Staphylococcus aureus treated with tetracycline (TC, 4MIC: 1.56 microg ml(-1)) was examined using the protein A-gold labelling method. No labelling was noted on the surface of the next septal region, but it was observed on the previous septal region. In contrast, on untreated cells the gold particles were found not only along the septal surface, but also on the next septal region. These findings agree with those obtained from both ultrathin and whole-mount specimens, indicating that TC inhibits the localization of ATL in the next septal region. They further support the suggestion that ATL localization on the cell surface is closely associated with the cell cycle. When S. aureus was treated with otherwise lytic concentrations of penicillin G (PCG, 4MIC: 0.02 microg ml(-1)) in the presence of TC, the distribution of the label on the cell surface was found to be very similar to that on the TC-treated organisms. This suggests that the inhibitory effect of TC on PCG-induced lysis was not due to complete loss of ATL on the cell surface but instead to inhibition of ATL localization in the next septal region.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have