Abstract
The ability of Superdex 200, a gel filtration matrix consisting of dextran-grafted beads, to act as a support for liquid-liquid partition chromatography (LLPC) in aqueous polyethylene glycol (PEG)-dextran two-phase systems was examined. The gel adsorbed the dextran-rich bottom phase readily and retained it during elution with the PEG-rich top phase. In contrast to LiParGel 650, a matrix designed for LLPC, the entire Superdex matrix seemed to form an immobilized stationary phase. Ideal partitioning of proteins was observed only for molecules partitioning towards the stationary phase on Superdex and for those favouring the mobile phase on LiParGel. Hence, the choice of matrix depends on the separation problem at hand.
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