Supporting data of spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo.

Gustavo A Higuera,Nils Aufferman,Vinod Subramaniam,Hugo Fernandes,Tim W.G.M Spitters,Johannes P Van Leeuwen,Lorenzo Moroni,Clemens Van Blitterswijk,Jeroen Van De Peppel,Jan De Boer,Reinout Stoop,Maryana Escalante,Marcel Karperien,Anton Van Boxtel,Roman Truckenmueller

doi:10.1016/j.dib.2015.08.012

Gustavo A Higuera, Nils Aufferman + Show 13 more

Open Access

https://doi.org/10.1016/j.dib.2015.08.012

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Abstract

This data article contains seven figures and two tables supporting the research article entitled: spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo[1]. The data explain the culture of stromal cells in vitro in three culture systems: discs, scaffolds and scaffolds in a perfusion bioreactor system. Also, quantification of extracellular matrix components (ECM) in vitro and staining of ECM components in vivo can be found here. Finally the quantification of blood vessels dimensions from CD31 signals and representative histograms of stanniocalcin-1 fluorescent signals in negative controls and experimental conditions in vivo are presented.

Highlights

This data article contains seven figures and two tables supporting the research article entitled: spatiotemporal proliferation of human stromal cells adjusts to nutrient availability and leads to stanniocalcin-1 expression in vitro and in vivo [1]
The data explain the culture of stromal cells in vitro in three culture systems: discs, scaffolds and scaffolds in a perfusion bioreactor system
Experimental factors Human stromal cells were cultured in 2D, 3D, under shear stress, concentrated in implantable wells and implanted in mice

Summary

Scaffold characterization

Microcomputed tomography (μCT, eXplore Locus SP μCT scanner, GE, Brussels, Belgium) at 14 μm resolution were used to characterize 2D and 3D scaffolds. Porosity, and surface area of 2D and 3D scaffolds were determined with Microview software (Open source) as performed before [2]. The threshold was adjusted to differentiate on the grayscale image between polymer voxels and pore voxels (One voxel was a 23 Â 23 Â 23 μm volume-element). The fraction of pore voxels within a scaffold determined its porosity. The pore size was determined by filling pore voxels with overlapping spheres [3]. The average size of a sphere occupying the pore voxel determined the average pore size. The boundaries between pore and polymer voxels determine the specific surface area.(Fig. 1 and Tables 1 and 2)

Perfusion bioreactor culture

Histological stainings