Abstract
The objective of this study was to test the efficiency of powdered coconut water (ACP-406®) base-medium without or with the addition of supplements on in vitro culture of isolated goat secondary follicles. Follicles were cultured for 18 days in α-MEM or in ACP-406®, both without supplements (referred to as α-MEM and ACP, respectively), or both supplemented with BSA, insulin, transferrin, selenium, glutamine, hypoxanthine, and ascorbic acid (referred to as α-MEM+ and ACP+). Follicular morphology, antrum formation, follicular and oocyte diameter, levels of glutathione (GSH), and chromatin configuration after in vitro maturation were evaluated. At the end of culture, ACP-406® base-medium (without or with supplements) showed a higher (P < 0.05) percentage of normal follicles than α-MEM (without or with supplements). Antrum formation was similar among α-MEM+, ACP and ACP+, and significantly higher than α-MEM without supplements. The follicular diameter was greater in ACP+ than α-MEM, and similar to other treatments. Moreover, fully and daily grown rates were higher (P < 0.05) in ACP-406® base-medium (without or with supplements) than α-MEM (without or with supplements). Levels of GSH were similar between ACP+ and α-MEM+ treatments. Both ACP+ and α-MEM+ allowed meiotic resumption without a significant difference between the two groups. In conclusion, supplemented ACP-406® base-medium maintained follicular survival and promoted the development as well as meiotic resumption of isolated goat secondary follicles cultured in vitro for 18 days.
Highlights
In vitro culture of ovarian follicles has emerged as a potential reproductive technology to produce large numbers of mature oocytes that are capable of fertilization (Demeestere et al, 2005)
Only ACP treatments maintained the follicular survival throughout the culture period
This study investigated for the first time the effect of ACP-406 base-medium on the in vitro development of goat secondary follicles followed by in vitro maturation of oocytes
Summary
In vitro culture of ovarian follicles has emerged as a potential reproductive technology to produce large numbers of mature oocytes that are capable of fertilization (Demeestere et al, 2005). This contributes to a better understanding of the mechanisms involved in oocyte development and control of folliculogenesis (Silva et al, 2016). The use of synthetic media could be expensive and less accessible. This has led researchers to investigate alternative and obtainable media such as powdered coconut water
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