Supplementation with Fetal-Specific Factors Ameliorates Oxidative Liver Damage During Hypothermic Storage and Reperfusion in a Rat Model

Abstract

The search for effective methods of long-term liver hypothermic preservation is one of the most important problems in hepatic transplantation. Biologically active substances contained in fetal tissues may be potent stimulators and regulators of metabolic processes, which can support liver during hypothermic storage and after normothermic reperfusion (HS/NR). The aim of this work was to investigate possible influences of fetal-specific factors (FSF) on liver pro-oxidant/antioxidant status, which frequently becomes disturbed during HS/NR in a rat model. As a source of FSF, human fetal tissue cytosolic fraction was used. At 4 h before liver isolation, FSF or Hank's solution were introduced into rat femoral vein (0.3 mL/100 g of body weight). Livers were subjected to HS at 4°C in sucrose-based solution (SBS) for 1 or 24 h, followed by NR for 60 min in vitro. After 1 and 24 h HS, FSF supplementation caused the normalization of hepatic thiobarbituric acid-reactive substances (TBARS) and their accumulation durin...