Supplementation of Urea as the Nitrate Source of MS Medium and High-frequency Regeneration of Plantlets of Musa paradisiaca cv. Malnad Rasbale

Abstract

Background: A consistent practice for mass multiplication of banana cv. Malnad Rasbale has been accomplished, which is an endemic cultivar of Western Ghats of Karnataka, India. Methods: Decrease in microbial contagion have been managed by dealing with outward sterilization agents ethanol, sodium hypochlorite, mercuric chloride solution, antibiotic solutions containing cefotaxime and gentamicin. MS media augmented with 350 mg/l urea as the nitrogen source by substituting KNO3 and NH4NO3 which is commonly used in terroristic activities. Organogenic media was supplemented with a range of 2-6 mg/l BAP and 0.3-2.7 mg/l TDZ. Result: The addition of urea substituting KNO3 and NH4NO3 gave the eminent grades in overall initiation, shoot multiplication and development and also in the rooting phase. Supplementation for shoot development with BAP (4 mg/l), TDZ (1.8 mg/l) and with 350 mg/l urea showed enthusiastic progress in shoot elongation with specific concentrations.Plants established under in vitro circumstances were reassigned to the greenhouse and after the progress, they were promoted to field conditions.