Supplementation of Red Fruit (Pandanus Conoideus) Extract Improved Lipid Profile, Decreased Concentration of F2 Isoprostane and Bodyweight in Male Wistar Strain (Rattus Norvegicus) Rats Given High-Fat Diet

Abstract

Backgrounds: Red fruit plant (Pandanus conoideus) that grows in eastern Indonesia, has the strong antioxidant properties, such as flavonoids, tannins, beta-carotene, vitamin C, and vitamin E. Study objectives to prove the antioxidant effect of red fruit extract inhibit dyslipidemia, the F2 Isoprostane concentration, the weight gain in animal with high lipid diet. Methods: The pre and posttest control group design experiment study was conducted on male Wistar rats. The subject was divided randomly into two groups. Both groups were administrated with hegh fat diet. The control group was blinded with 1 mL aquadestylates, and the treatment group, which was treated with 168 mg red fruit extract in 1 mL for 30 days. Dependent variables; lipid profile, levels of F2 isoprostane, and bodyweight were measured before and after intervention. Results: We found the improvement of lipid profile in the treatment group; decreased of LDL cholesterol (p<0.001), triglycerides (p<0.001), total cholesterol (p<0.001), and increased HDL cholesterol (p<0.001). Other wised there was no change of lipid profile in the control group. F2 Isoprostane was also decreased (p<0.001) in the treatment group, but not in the control group. The bodyweight was decreased in the treatment goup (p<0.001), but in contrary increased in the control group (p<0.001). Conclusion: Supplementation of red fruit extract (Pandanus conoideus) significantly improved the lipid profile, decreased levels of F2 isoprostane, as well as bodyweight on male Wistar strain (Rattus norvegicus) rats that was administrated with a high-fat diet Keywords: antioxidant, red fruit extract, lipid profile, F2 Isoprostane, body weight