Abstract
The supplementation of culture medium with platelet activating factor (PAF) on the subsequent implantation and pregnancy potential of pre-embryos produced by in-vitro fertilisation was studied. Pre-embryo culture medium was supplemented with 0 (control), 0·186, 0·93, or 1·49 μmol/l PAF. Pre-embryos were transferred to PAF-containing medium 15-17 h after insemination (ie, just before syngamy) for 24 h and then transferred to the uterus. For 185 women receiving control pre-embryos, the pregnancy rate (positive beta human chorionic gonadotropin per oocyte retrieval) was 10·2%, while 166 women who received PAF treated pre-embryos (all concentrations combined) achieved a pregnancy rate of 17·5%. This difference was significant. The pregnancy rates per pre-embryo transferred were 6·1% and 9·4% for the control and PAF groups, respectively. The percentage of positive pregnancy tests that resulted in a viable pregnancy (presence of fetal heart at 8 weeks) was 78·9% in the controls and 75·9% in the PAF group. There was no difference in the average number of embryos transferred in either group. The increase in the pregnancy rate after a short exposure of pre-embryos to PAF in vitro suggests that PAF mediates pre-embryo development.
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