Supplementation of curcuma domestica extract reduces cox-2 and inos expression on raw 264.7 cells

Abstract

The expression of COX-2 and iNOS have an important role in inflammation. Turmeric (Curcuma domestica) contains active compounds such as curcumin, which can inhibit the expression of the enzyme so that the risk of chronic inflammation and cancer can be reduced. The aim of this study was to determine the anti-inflammatory effects of turmeric extract concentrations on the expression of COX-2 and iNOS in RAW 264.7 cells induced by lipopolysaccharide (LPS). This study was an experimental post-test only with a control group design. The RAW 264.7 cultured cells were divided into six groups (G) on a 24-well plate; GA (control group without LPS), GB (control group with LPS), GC (control group without primary antibody), GD (62,5μg/mL extract concentration), GE (125μg/mL extract concentration), and GF (250μg/mL extract concentration). After 18 hours of treatment, all the cells were fixed with ethanol and stored and tested with immunocytochemistry. Results show the expressions of COX-2 and iNOS in GA (10,51±5,15; 12,51±3.10), GB (82,29±1,49; 82,70±1,67), GC (29,01±5,19; 16,33±1,61), GD (32,19±5,36; 58,58±10,31), GE (24,29±5,88; 52,55±9,03), and GF (40,42±3,15; 29,24±7,84). The test results of data analysis by one-way ANOVA (COX-2) and Kruskal-Wallis (iNOS) showed significant differences (p <0.05) along with Post Hoc test Tamhanes (COX-2) and Mann-Whitney (iNOS), which also showed significant differences (p <0.05). The most substantial differences shown by the mean of the treatment group turmeric extract concentration were 125 μg/mL (COX-2) and 250μg/mL (iNOS). These results suggest that turmeric extracts can reduce the effects of inflammation on RAW 264.7 cells induced by lipopolysaccharide