Supplementary Figures S1-S12 from Chromoplexy Is a Frequent Early Clonal Event in <i>EWSR1</i>-Rearranged Round Cell Sarcomas That Can Be Detected Using Clinically Validated Targeted Sequencing Panels

Abstract

<p>Figure S1. gGraphs depicting gWalk showing connectivities of chromoplectic structural variants (each represented by different color paths) to common nodes. Figure S2. Circos plots depicting the canonical and noncanonical structural variants detected in prostate carcinoma cases with TMPRSS2::ERG driver fusion. Figure S3. Inter- vs. intrachromosomal chromooplectic events among cases with EWSR1::FLI1, EWSR1::ERG or EWSR1::WT1 driver fusions. Figure S4. Longitudinal sequencing of sequential Ewing sarcoma (EWSR1::FLI1) cases illustrating structural variants, gene-level mutations and copy number variations, and arm-level chromosomal gains and losses. Figure S5. Longitudinal sequencing of sequential desmoplastic small round cell tumor (EWSR1::WT1) cases illustrating structural variants, gene-level mutations and copy number variations, and arm-level chromosomal gains and losses. Figure S6. Distribution of age among cases with EWSR1::FLI1, EWSR1::ERG or EWSR1::WT1 with or without chromoplexy. Figure S7. Heatmap and boxplot quantifying percentage genome with copy number variations among cases with EWSR1::FLI1, EWSR1::ERG or EWSR1::WT1 with or without chromoplexy. Figure S8. Frequency of chr8 gains among cases with EWSR1::FLI1, EWSR1::ERG or EWSR1::WT1 with or without chromoplexy. Figure S9. Kaplan-Meier curves for overall and progression-free survival in Ewing sarcoma with EWSR1::FLI1 versus EWSR1::ERG. Figure S10. Kaplan-Meier curves for overall and progression-free survival in Ewing sarcoma with or without chromoplexy. Figure S11. Kaplan-Meier curves for overall and progression-free survival in desmoplastic small round cell tumor with or without chromoplexy. Figure S12. Kaplan-Meier curves for overall and progression-free survival in all Ewing sarcoma cases with or without chr8 gains.</p>