Supplementary Figures 1-8, Supplementary Materials and Methods from Epigenetic Silencing of BMP6 by the SIN3A–HDAC1/2 Repressor Complex Drives Melanoma Metastasis via FAM83G/PAWS1

Abstract

<p>S1. Kaplan-Meier survival analysis of patients expressing high and low levels of BMP family genes. S2. Kaplan-Meier PFS analyses of cutaneous melanoma patients with BRAF-mutant and wild-type melanomas. S3. Kaplan-Meier OS analyses of cutaneous melanoma patients with BRAF-mutant and wild-type melanomas. S4. ChIP-PCR analysis demonstrating the binding of SIN3A-HDAC1/2 repressor complex in the proximal promoter regions of BMP1, BMP2 and BMP6 genes in 1205Lu melanoma cells. S5. Physiological changes of melanoma cells with stable expression of BMP6. S6. Establishment of mouse lung-specific metastatic melanoma cells for metastasis model generation. S7. Transcriptionally decreased FAM83G by BMP6 suppresses invasion capacity of melanoma cells via F-Actin deregulation. S8. Evaluation of functional roles of FAM83G in a metastasis model.</p>