Supplementary Figures 1-5 from GR and ER Coactivation Alters the Expression of Differentiation Genes and Associates with Improved ER<sup>+</sup> Breast Cancer Outcome

Abstract

<p>Supplementary Figure 1. ChIP-seq for GR and ER peaks and associated genes and motifs. Supplementary Figure 2. GR immunoprecipitation demonstrates ER interaction. Supplementary Figure 3. Most represented transcription factor response element motifs in Dex/E2-treated EBR and GBR peaks in regulatory regions of KDM4B, IGFBP4, and VDR. Supplementary Figure 4. Knockdown efficiency at 24h and 48h as measured by Q-RT-PCR, normalized to control siRNA pool. Supplementary Figure 5. Flow chart of GR ChIP-seq data analysis and integration of genome-wide gene expression (microarray) data.</p>