Supplementary Figures 1-5 from Extensive Promoter DNA Hypermethylation and Hypomethylation Is Associated with Aberrant MicroRNA Expression in Chronic Lymphocytic Leukemia

Abstract

<p>PDF file - 90K, Supplementary Figure S1 contains technical validations of differentially methylated miRNA promoters by high resolution quantitative DNA methylation analysis. Supplementary Figure S2 shows experimental validation of promoter activity for the mir-129-2 locus. Supplementary Figure S3 shows relative expression of predicted miRNA target genes by qPCR in CLL specimens and healthy B cell controls. Supplementary Figure S4 shows an unsupervised clustering of quantitative DNA methylation data at the identified hyper- and hypomethylated miRNA promoters in CLL specimens, healthy B cell samples, various normal hematopoietic cells and several solid tissues. Supplementary Figure S5 shows a detailed quantitative DNA methylation analysis of the miR-155 host gene 5' region and correlations with miR-155 expression in CLL specimens, healthy B cell samples, various normal hematopoietic cells and several solid tissues</p>