Supplementary Figures 1 through 7, Supplementary Tables 1 through 9 from Combined <i>MYC</i> Activation and <i>Pten</i> Loss Are Sufficient to Create Genomic Instability and Lethal Metastatic Prostate Cancer

Abstract

<p>Supplementary Figure 1 Morphology of PIN in B13-MYC mice. Supplementary Figure 2 Phenotypic analysis of PIN in a B13-MYC mouse (ventral lobe, 52 weeks old). Supplementary Figure 3 Additional phenotypic analysis of B-MYC/Pten mouse PIN (anterior lobe, 8 weeks). Supplementary Figure 4 Invasive adenocarcinoma lesion in a 14 week old B-MYC/Pten animal. Supplementary Figure 5 Chromogranin (a) staining in a primary tumor and Foxa2 (b) staining in a lymph node metastasis from a 24 week-old B-MYC/Pten animal shows focal scattered tumor cells staining positively (arrows). Supplementary Figure 6 Morphological features of castrated B-MYC/Pten animals. Supplementary Figure 7. Genes within regions of copy number gains and losses are enriched in specific gene ontology (GO) gene sets. Supplementary Table 1. Prostate Phenotype in B-MYC/PtenFl/Fl mice and wild type (FVB/N) mice. Supplementary Table 2. Metastatic Spread in B-MYC/PtenFl/Fl mice and wild type (FVB/N) mice. Supplementary Table 3. Prostate Phenotype in B-MYC/PtenFl/+ mice and wild type mice. Supplementary Table 4. Effects of androgen withdrawal/castration in B-MYC/PtenFl/Fl mice and wild type (FVB/N) mice. Supplementary Table 5. Metastatic Spread in B-MYC/PtenFl/Fl mice after androgen withdrawal. Supplementary Table 6. Somatic copy number gains and losses in each sample. Supplementary Table 7. Regions of Copy Number alterations in each sample. Supplementary Table 8. Regions with recurrent gains and losses in at least two mice. Supplementary Table 9. Regions of high copy number gain and low copy number loss in any tumor specimen.</p>