Supplementary Figures 1 - 8 from Nucleoside Diphosphate Kinase-3 (<i>NME3</i>) Enhances TLR5-Induced NF<b>κ</b>B Activation

Abstract

<p>Supplementary Figures 1-8. Figure S1. Dosed-dependent activation of transcriptionally-activated κB5:FLuc reporter by flagellin and heat-killed Salmonella in HCT116 colon carcinoma cells. Figure S2. Inhibition of the transcriptionally-coupled κB5:IκBα-FLuc fusion reporter by shTLR5 is rescued by increasing the flagellin concentration. Figure S3. High-throughput screening data. Normalized photon flux data for 691 targeted kinases is shown at 45 min (a) and 245 min (b) after Salmonella stimulation. Figure S4. NME3 regulates TLR5-induced NF-κB signaling. Figure 5S. TLR5 mRNA levels correlate with overall patient survival in various cancers. Figure S6. NF-κB1 mRNA levels significantly correlate with enhanced patient survival in breast cancer. Gehan-Breslow-Wilcoxon test were used to estimate the p-values between high and low expression groups (using top and bottom 25% as the cutoff for grouping). Figure S7. CDK6 mRNA levels correlate with overall poorer patient survival in various cancers. Gehan-Breslow-Wilcoxon test were used to estimate the p-values between high and low expression groups (using top and bottom 25% as the cutoff for grouping). Figure S8. NME3 exhibits mutations within or adjacent to the kinase-active site in various cancers.</p>