Supplementary Figures 1 - 7 from Plasma MicroRNAs as Novel Biomarkers for Endometriosis and Endometriosis-Associated Ovarian Cancer

Abstract

<p>PDF file - 488K, Supplementary Figure 1: Experimental design. Supplementary Figure 2. Reliability of RNA extraction and reproducibility of RTqPCR techniques. Supplementary Figure 3. Reliability of RT-qPCR assays. Supplementary Figure 4. miR-132 demonstrates consistent CT values (ranging from 27 to 29.5.) across all categories of plasma samples (healthy 0=Normal Healthy Endo1=mEnedtoriosis 2 = SSOerCous 3E=EAAOOCC controls, n=20; endometriosis, n=33; EAOC, n=14; and SOC, n=21), and was used for normalization of RT-qPCR results in this study. Supplementary Figure 5. Unsupervised hierarchical clustering analysis of samples based on the 23-miRNA expression profiles. Supplementary Figure 6. Box plots of top three differentially expressed miRNAs in pair-wise comparisons. Supplementary Figure 7. Lack of correlation for miRNA expression between matching tumor tissue and plasma samples from five tumor-bearing LSLKrasG12D/+/ Ptenloxp/loxp mice.</p>