Supplementary Figure S5: Elevated ATF3 after AD and edelfosine treatments inhibits AR promoter activity in LNCaP cells. from Edelfosine Promotes Apoptosis in Androgen-Deprived Prostate Tumors by Increasing ATF3 and Inhibiting Androgen Receptor Activity

Abstract

<p>Androgen deprived LNCaP cells were co-transfected with 5 µg of ARE-luciferase reporter constructs, renilla luciferase and siATF3 (10 nM), followed by treatment with edelfosine (5 µM) and R1881 (5 nM). Cells were harvested and lysed after 24 hours following treatment and assayed for ARE reporter activity. Luciferase expression of each sample was measured using dual luciferase reporter gene assay system (Promega Corp., Madison, WI). The data represents the ratio between firefly/renilla luciferase activities. The mean values {plus minus} SEM of three independent experiments are shown. *p < 0.05, compared to group with R1881 alone (one way ANOVA).</p>