Abstract

<p>PDF - 62KB, Supplementary Figure 2. PRIMA-1Met induces transcriptional regulation of a set of apoptosis target genes. (A) Microarray analysis of gene expression in MM.1S, U266, and 8266R5 cells after 8 hrs treatment with PRIMA-1Met. The heat map of genes differentially expressed in 3 MM cell lines in duplicates. Vertical rows indicate separate arrays, and horizontal rows indicate genes. Values are normalized to untreated control. Green indicates low expression; red indicates high expression. (B) mRNA levels of some of the selected genes modulated by PRIMA-1Met were detected by qRT-PCR in 3 MM cell lines treated similarly with PRIMA-1Met. Values were normalized to those obtained for glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and expressed as log2-fold induction over DMSO-treated cells (mean plus or minus SD).</p>

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