Supplementary Figure 1-8 from Calcium Promotes Human Gastric Cancer via a Novel Coupling of Calcium-Sensing Receptor and TRPV4 Channel

Abstract

<p>Supplementray Figure 1. Effect of CaCl2 on PCNA protein expression in GC cells and xenografed gastric tumors. Supplementray Figure 2. The efficiencies of CaSR shRNA transient transfection into MKN45 cells and CaSR shRNA lentiviruses transfection into SGC-7901 cells. Supplementray Figure 3. Comparisons of serum calcium concentrations in nude mice without or with an intravenous injection of CaCl2 into the tail veins. Supplementary Figure 4. CaSR-mediated Ca2+o entry through the ROC in GC cells. Supplementary Figure 5. CaSR-induced phosphorylation of β-caternin and PI3K/AKT signaling pathways in cancer cells. Supplementary Figure 6. CaSR-mediated ERK1/2, JNK signaling pathways in human normal gastric cells and cancer cells. Supplementary Figure 7. CaSR-induced phosphorylation of ERK and JUK signaling pathways and proliferation, migration and invasion of cancer cells. Supplementary Figure 8. Effect of TRPV4 agonist or antagonist on p-AKT and p-β-caternin and CaCl2-induced migration of cancer cells.</p>