Supplementary Data from Targeting the Temporal Dynamics of Hypoxia-Induced Tumor-Secreted Factors Halts Tumor Migration

Abstract

<p>Supplementary Information includes supplemental experimental procedures, description of gene regulatory network, two tables, eight figures and a supplementary video. Supplementary Table S1. List of primers used Supplementary Table S2. Parameters used in the ODEs Supplementary Figure S1: Large T47D microtumors exhibit collective migration, hypoxia and aggressive phenotype. Supplementary video showing migration of a microtumor Supplementary Figure S2: Treatment of 150 and 600μm microtumors with conditioned media of 150μm microtumors (150/CM). Supplementary Figure S3: Morphology and multi-parameter flow cytometry on breast cancer metastatic pleural effusion cells. Supplementary Figure S4: Large microtumors of patient-derived primary cancer cells and xenograft cells showed migratory behavior. Supplementary Figure S5: Integrated regulatory network of tumor secretome factors and signaling molecules involved in microtumor migration. Supplementary Figure S6: HIF-1α inhibition at later time points does not inhibit migration of 600μm microtumors. Supplementary Figure S7: Effect of MMP inhibitor treatment on the collective migration of large microtumors of T47D cell line and patient-derived metastatic breast cancer (mBC) cells. Supplementary Figure S8: Effect of Anti-sE-CAD antibody treatment on the collective migration of large microtumors of T47D cells and patient-derived metastatic breast cancer (mBC) cells.</p>