Supplementary Data from Pharmacologic Ascorbate Primes Pancreatic Cancer Cells for Death by Rewiring Cellular Energetics and Inducing DNA Damage

Abstract

<p>S1. There is differential susceptibility to P-AscH- across cell lines. S2. Bolus addition of hydrogen peroxide depletes intracellular ATP and NAD+ in MIA PaCa-2 and PANC-1 cells. S3. Pharmacological ascorbate depletes intracellular NADH in MIA PaCa-2 cells, but not in PANC-1, 339, and H6c7 cells. S4. Pharmacological inhibition of PARP1 by olaparib enhances the cytotoxicity of P-AscH- in MIA PaCa-2 cells. S5. A combination of P-AscH- and prexasertib synergistically inhibits cell proliferation of MIA PaCa-2 cells. Supplementary Table S1. Normal pancreatic cells are more resistant to P-AscH- than pancreatic cancer cells. Supplementary Table S2. The ED50's for depletion of ATP and NAD+ correlate with kcell for the removal of H2O2. Supplementary Table S3. The gene target and primer pairs used for QPCR.</p>