Supplementary Data from Eradication of Large Solid Tumors by Gene Therapy with a T-Cell Receptor Targeting a Single Cancer-Specific Point Mutation

Abstract

<p>Supplementary Methods - Contains detailed information on whole exome and RNA sequencing, variant calling, TCR gene transfer, and longitudinal imaging Supplementary Fig. S1 - Pipeline for exome/RNA analysis and neoepitope prediction Supplementary Fig. S2 - Pipeline for exome and clustering analysis of autochthonous 8101 tumor fragments Supplementary Fig. S3 - Generation of 1D9 T cells Supplementary Fig. S4 - Generation of mp68-expressing tumor cell lines Supplementary Fig. S5 - Expansion of 1D9 T cells is antigen-specific and not driven by lymphopenia-induced proliferation Supplementary Fig. S6 - 1D9 T cells do not infiltrate in tumors if mp68 is not expressed Supplementary Table S1 - Results of whole exome sequencing of Bulk tumor cells and Bulk reisolates after 1D9 T cell therapy Supplementary Table S2 - Results of whole exome sequencing of fragments derived from the autochthonous 8101 tumor</p>