Supplementary Data from Direct Targeting of <i>MYCN</i> Gene Amplification by Site-Specific DNA Alkylation in Neuroblastoma

Abstract

<p>Supplementary Figure S1. MYCN-amplified neuroblastoma cell lines are addicted to MYCN. Supplementary Figure S2. Cytotoxic evaluation of PI polyamides targeting the MYCN gene. Supplementary Figure S3. Direct alkylation of the targeted DNA of MYCN gene. Supplementary Figure S4. MYCN-A3 and MYCN-A4 inhibit MYCN expression and cell proliferation in MYCN-amplified neuroblastoma cell lines. Supplementary Figure S5. Global impact of MYCN-A3 on gene expression in MYCN-amplified neuroblastoma cells is comparable to that of CRISPR/Cas9 targeting MYCN. Supplementary Figure S6. MYCN-A3 induces cell cycle arrest and the expression levels of p53 family genes in neuroblastoma cell lines. Supplementary Figure S7. MYCN-A3 suppresses tumor growth in human neuroblastoma xenograft mouse models. Supplementary Figure S8. Pharmacological effects of MYCN-A3 on human MYCN-amplified neuroblastoma xenograft models in vivo. Supplementary Figure S9. MYCN-A3 affects the MYCN gene loci and not non-targeted gene loci.</p>